Mutational Signatures (v3.2 - March 2021)
Somatic mutations are present in all cells of the human body and occur throughout life. They are the consequence of multiple mutational processes, including the intrinsic slight infidelity of the DNA replication machinery, exogenous or endogenous mutagen exposures, enzymatic modification of DNA and defective DNA repair. Different mutational processes generate unique combinations of mutation types, termed “Mutational Signatures”.
In the past few years, large-scale analyses have revealed many mutational signatures across the spectrum of human cancer types, including the latest effort by the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Network (Alexandrov, L.B. et al., 2020 ) using data from more than 23,000 cancer patients.
COSMIC Mutational Signatures is a collaboration between Wellcome Sanger Institute, Cambridge, UK, COSMIC and the Alexandrov lab at the University of California, San Diego, USA, part of the wider Cancer Grand Challenges Project.
As the number of mutational signatures and variant classes considered has increased, the need for a curated census of signatures has become apparent. Here, we deliver such a resource by providing a comprehensive overview of the key information known, suspected or widely discussed in the scientific literature for each of the identified mutational signatures on a dedicated website.
This summary includes the mutational profile, proposed aetiology and tissue distribution of each signature, as well as potential associations with other mutational signatures and how the signature has changed during iterations of analysis.
Currently, three different variant classes are considered, resulting in the following sets of mutational signatures.
Download current COSMIC Mutational Signatures version 3.2 and previous releases here.
Mutational signatures version 3 was released as part of COSMIC release v89 (May 2019), updated to version 3.1 in COSMIC release v91 (June 2020), and to version 3.2 in COSMIC release v93 (March 2021). Version 2 signatures (March 2015) that were part of earlier COSMIC releases can still be consulted.
The current set of mutational signatures has been extracted using SigProfiler, a compilation of publicly available bioinformatic tools addressing all the steps needed for signature identification. SigProfiler functionalities include mutation matrix generation from raw data and signature extraction, among others.
Mutational signatures as a collection of operative mutational processes
Mutational processes from different aetiologies are active during the course of cancer development. They can be identified using mutational signatures, due to their unique mutational pattern and specific activity on the genome.
This is illustrated in the figure below using a framework of 6 classes of single base substitutions, and three distinct mutational processes, whose respective strengths vary throughout a patient’s life. At the beginning, all mutations were due to the activity of the endogenous mutational process. As time progresses, the other processes get activated and the mutational spectrum of the cancer genome continues to change.